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human library plasmid dna pools  (Cellecta Inc)

 
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    Cellecta Inc human library plasmid dna pools
    Human Library Plasmid Dna Pools, supplied by Cellecta Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human library plasmid dna pools/product/Cellecta Inc
    Average 90 stars, based on 1 article reviews
    human library plasmid dna pools - by Bioz Stars, 2026-03
    90/100 stars

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    The gRNA-encoding plasmid DNA in the pooled CRISPR library contained the nucleotide sequence 5’-CGG-3’, which could be used as the PAM sequence for Cas9 proteins. Cas9 RNPs, which are complexes of Cas9 proteins and rc-gRNAs, could selectively cleave plasmid DNA possessing complementary sequences with rc-gRNA. Based on this principle, only the desired gRNAs could be depleted from the pooled CRISPR library.

    Journal: PLoS ONE

    Article Title: CRISPR/Cas-based customization of pooled CRISPR libraries

    doi: 10.1371/journal.pone.0199473

    Figure Lengend Snippet: The gRNA-encoding plasmid DNA in the pooled CRISPR library contained the nucleotide sequence 5’-CGG-3’, which could be used as the PAM sequence for Cas9 proteins. Cas9 RNPs, which are complexes of Cas9 proteins and rc-gRNAs, could selectively cleave plasmid DNA possessing complementary sequences with rc-gRNA. Based on this principle, only the desired gRNAs could be depleted from the pooled CRISPR library.

    Article Snippet: For generating the gRNA-depleted library, 20 μg of pooled CRISPR library plasmids DNA (human GeCKO v1 and v2 lentiviral sgRNA libraries, Addgene #1000000049) was incubated with NEBuffer 3.1 containing 1× Cas9 RNPs and a complex of 10 μg of Cas9 proteins and 7.5 μg of rc-gRNAs for 8 h at 37°C.

    Techniques: Plasmid Preparation, CRISPR, Sequencing